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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Bone Miner Res. 2012 Sep;27(9):2001–2014. doi: 10.1002/jbmr.1663

Figure 2. PTH antagonizes inhibition effect of noggin on phosphorylation of Smad1.

Figure 2

(A,B) PTH enhanced BMP2-stimulated phosphorylation of Smad1 in a dose (A) and time (B) dependent manner in osteoblastic UMR106 cells.

(C) PTH promoted and prolonged BMP2-stimulated phosphorylation of p38 in a time-dependent manner.

(D,E) PTH enhanced BMP2-stimulated phosphorylation of Smad1 in C2C12 cells (D) and GFP-labeled Sca-1+CD45−CD11b− MSCs (E) and noggin inhibited the effect of both as shown by Western blot analyses with Abs to phosphor-Smad1/5/8 and Smad1 with quantification by densitometry shown directly below each lane. Data are presented as mean ± SEM. n = 3. *p < 0.05 versus BMP2 alone; #p < 0.05 versus BMP2 and noggin.

(F) PTH failed to enhance levels of pSmad1 in OC-59 PTH1R-deficient cells.