Table 1.

Phenotypes obtained upon single or double injections of different hmcn2 and fbln1 morpholinos

stage	30hpf		55hpf
Phenotype	Blisters trunk	n	Blisters trunk	Fin defect	n
MO
hmcn2 0.5 mM	0%	163	0%	0%	163
fbln1 0.1 mM	0%	176	0%	0%	176
fbln1c 0.125 mM	0%	97	0%	0%	97
fbln1d 0.125 mM	0%	98	0%	0%	98
hmcn2 0.5mM + fbln1 0.1 mM	60,4%	447	37%	62,7%	243
hmcn2 0.5 mM + fbln1c 0.125 mM	0%	93	0%	74,2%	93
hmcn2 0.5 mM + fbln1d 0.125 mM	0%	120	0%	63,3%	120
hmcn2 0.5 mM + fbln1c 0.125 mM+ fbln1d 0.125 mM	61,3%	132	41%	84,8%	132

Defects were analyzed via light microscopy at indicated stages. The reduction of trunk blistering frequency from 30 hpf to 55 hpf is caused by progressive healing of this phenotypic trait (see Figure 2 and Supplementary Fig. S5C,E for 72 hpf), whereas fin defects persist (see Supplementary Fig. S5D,F for 72 hpf). For testing of Fbln1 splice variants, note that fin defects were obtained both upon co-injection of fbln1c and hmcn2 MOs, and upon co-injection of fbln1d and hmcn2 MOs, whereas trunk blistering required triple injection of fbln1c, fbln1d and hmcn2 MOs. n, number of scored embryos (3 independent experiments).