Figure 1. Inhibition of TRPV3-mediated current by [Mg²⁺]_o in cultured mouse keratinocytes.

(a) Representative current traces in response to voltage ramp from -100 mV to +100 mV without (basal) and with the TRPV3 agonist cocktail in the absence (0 mM) and presence of 2 and 10 mM Mg²⁺. (b) Percentage of inhibition of TRPV3 agonist cocktail-activated current by 10 mM Mg²⁺ at holding potential of -100 mV and +100 mV obtained from the voltage ramp shown in (a). Please note the different inhibitory effects at -100 mV and +100 mV. (c) Rectification ratio [-(I₁₀₀/I_-100)] with different concentrations of Mg²⁺ in the intracellular and extracellular solutions. ** p<0.01 compared with 2 mM Mg²⁺ in the extracellular solution with 2 or 10 mM Mg²⁺ in the intracellular solution. ## p<0.01 compared with 2 mM Mg²⁺ in the intracellular solution with 0, 2, or 10 mM Mg²⁺ in the extracellular solution.