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. Author manuscript; available in PMC: 2013 Feb 25.
Published in final edited form as: Virology. 2012 Jun 18;432(2):283–295. doi: 10.1016/j.virol.2012.05.028

Fig. 1. Adenoviral protein V regulates subcellular localization of NPM1 during infection.

Fig. 1

HPAEC cells were infected with adenovirus at an MOI of 10 PFU/cell for various times, followed by immunofluorescent staining. Subcellular localization of adenoviral pV and endogenous NPM1 was observed in infected cells at various times post-infection. Viral and endogenous cellular proteins in infected cells were visualized by staining with anti-pV and anti-NPM1 followed by secondary antibody conjugated with fluorescent probes. Uninfected and infected cells were stained for nuclear DNA using Hoechst 33342 and fluorescent signals were visualized by epifluorescence microscopy.