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. Author manuscript; available in PMC: 2013 Jul 31.

Published in final edited form as: Cell Oncol (Dordr). 2012 Jan 31;35(2):95–110. doi: 10.1007/s13402-011-0068-y

Fig. 1 — uPA activity and uPAR expression in NSCLC. A. (Upper Panel) Conditioned media from each cell line was collected and assayed for uPA activity. n=6, no statistical difference among all three groups was determined using student a one way anova. (Lower Panel) Whole cell lysates from each cell line were collected and 50 μg of each lysate was subjected to SDS-PAGE. Expression of uPA receptor was determined with anti-UPAR antibodies. B. Recombinant purified uPA was incubated with vehicle (0.1% DMSO) or the indicated concentration of EIPA in 0.1% DMSO for 2 hours (white columns). Cultures of H460 were treated with EIPA (10μM) for 24 hours before collecting conditioned medium (grey columns). uPA activity was determined using a tripeptide substrate conjugated with a pNA. Statistical analysis was determined using a one tailed type one student T-test vs control, n=3.

Fig. 1 — uPA activity and uPAR expression in NSCLC. A. (Upper Panel) Conditioned media from each cell line was collected and assayed for uPA activity. n=6, no statistical difference among all three groups was determined using student a one way anova. (Lower Panel) Whole cell lysates from each cell line were collected and 50 μg of each lysate was subjected to SDS-PAGE. Expression of uPA receptor was determined with anti-UPAR antibodies. B. Recombinant purified uPA was incubated with vehicle (0.1% DMSO) or the indicated concentration of EIPA in 0.1% DMSO for 2 hours (white columns). Cultures of H460 were treated with EIPA (10μM) for 24 hours before collecting conditioned medium (grey columns). uPA activity was determined using a tripeptide substrate conjugated with a pNA. Statistical analysis was determined using a one tailed type one student T-test vs control, n=3.