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. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Cell Oncol (Dordr). 2012 Jan 31;35(2):95–110. doi: 10.1007/s13402-011-0068-y

Fig. 6.

Fig. 6

uPAR stimulation of MMP9 activity requires NHE1. Serum-starved H460 cells were stimulated in the absence or presence of either 10nM uPA or ATF for 16 hours. Where indicated, cells were treated with the NHE1 inhibitor EIPA for the duration of the experiment. After 24 hours, the conditioned medium was collected and analyzed for MMP9 activity by the hydrolysis of gelatin using a zymogen gel assay. Zymography is a representative gel. Bars represent the average of n=6 +/− SEM. Data were analyzed with a one-way Anova using Tukey’s Multiple Comparison with a 99.9% confidence interval between indicated groups (P-value of < 0.001).