Figure 2. VasaCre-mediated deletion of Sin3A leads to a loss of germ cells.

(a.) Representative immunostaining with an anti-TRA98 antibody on cross sections from D10, D14, D21 day old and adult testis from Sin3A^gc−/− and Sin3A^gc+/− mice. Scale bar =50 μm. Inset boxes correspond to higher magnification. (b.) Percentage of seminiferous tubules with positive staining for TRA98. Shown is the average result from two independent experiments (c-d.) Relative expression determined by quantitative RT-PCR in of Sin3A^gc+/− and Sin3A^gc−/− testes at indicated time points for (c) germ cell specific transcripts MVH, plzf, RBM, and ngf and (d) proliferation marker pcna. Transcript abundance is expressed as relative to housekeeping B2M transcript abundance and normalized to the corresponding Sin3A^gc+/− testes. Shown is the average result from two independent experiments performed in duplicate.