Figure 4. Sin3A mediated loss of germ cells affects Sertoli cells.

(a.) Representative immunostaining on cross sections of testes from D10, D14 and D21 mice of the indicated genotype with an anti-GATA1 antibody. Scale bar =50 μm. Inset boxes correspond to higher magnification (b.) Relative expression of Sertoli cell-specific transcripts, determined by quantitative RT-PCR in testis of Sin3A^gc+/− and Sin3A^gc−/− mice at indicated time points. Expression is relative to housekeeping gene B2M and normalized to corresponding Sin3A^gc+/− testis. Shown is the average of two independent experiments performed on two independent mice per genotype in duplicate.