Fig. 6.

Effects of various hormones on FGF9 gene expression in GC of small follicles. A, Effect of 2 d treatment of LH (30 ng/ml) and/or T₄ (100 ng/ml) on FGF9 mRNA abundance in SMGC (experiment 6). Cells were concomitantly treated with 30 ng/ml IGF-I and 30 ng/ml FSH. B, Effect of 24 h treatment of cortisol (300 ng/ml), PGE2 (300 ng/ml), SHH (500 ng/ml), WNT3A (300 ng/ml), and ANG (300 ng/ml) on FGF9 mRNA abundance in IGF-I-treated [control (Con)] SMGC (experiment 7). Values are means of three separate experiments (± sem) and normalized to constitutively expressed 18S rRNA. Within a panel, means without a common letter differ (P < 0.05).