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. 2012 Jul 13;153(9):4328–4335. doi: 10.1210/en.2012-1241

Fig. 1.

Fig. 1.

Effect of A-II and calcium ionophore on mRNA and protein expression of KCNJ5. A and D, Confluent cells were serum deprived in DMEM-F12 containing 0.1% cosmic calf serum for 24 h and then incubated with fresh media with 0.1% serum and no secretagogue, A-II (10 nm), or calcium ionophore A23187 for 3 h. After aspiration of media, the cells were collected for RNA extraction and real-time RT-PCR was performed. Results were normalized by GAPDH mRNA expression and expressed as fold change vs. control. B and C, Cells were serum deprived 72 h after infection in DMEM-F12 containing 0.1% cosmic calf serum for 24 h and then incubated with fresh media with 0.1% serum and no secretagogue or A-II (10 nm) for 8 h. After aspiration of media, the cells were harvested with protease inhibitor. The cell lysates were subjected to immunoblotting analysis using anti-KCNJ5 or antitubulin antibodies. The KCNJ5 band intensity of scanned images was adjusted by that of tubulin and expressed as fold change vs. control. *, P < 0.05 vs. control, n = 3; **, P < 0.01 vs. control, n = 3.