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. 2012 Jul 13;153(9):4328–4335. doi: 10.1210/en.2012-1241

Fig. 3.

Fig. 3.

Effect of shRNA-KCNJ5 on mRNA and protein expression of KCNJ5. Cells were serum deprived 72 h after transduction with control or either shRNA-KCNJ5 lentiviruses as in Fig 1. A, After aspiration of media, the cells were collected for RNA as in Fig 1. B and C, After aspiration of media, the cells were harvested with protease inhibitor. The cell lysates were subjected to immunoblotting analysis using anti-KCNJ5 or antitubulin antibodies. The KCNJ5 band intensity of scanned images was adjusted by that of tubulin and expressed as fold change vs. control. *, P < 0.01 vs. control, n = 3.