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Basal and A-II-stimulated aldosterone and cortisol production by HAC15 transduced with control, shRNA-KCNJ5 (A), or KCNJ5 lentiviruses (B, C). Cells were serum deprived 72 h after infection in DMEM-F12 containing 0.1% cosmic calf serum for 24 h after which cells were incubated with no secretagogue or A-II (10 nm) for 24 h. *, P < 0.05 vs. control, n = 4, **, P < 0.01 vs. control, n = 4.
