Fig. 1.
Gsα expression in PVNGsKO mice. A, In situ hybridization with antisense Gsα-specific probe in PVN of control, mPVNGsKO, and pPVNGsKO mice. Sense indicates the result of a control mouse using sense Gsα (dark field, upper panels; hematoxylin and eosin, lower panels). B and C, Quantitation of Gsα expression in PVN (panel B) and supraoptic nucleus (SON, panel C) by in situ hybridization in control (open bars), mPVNGsKO (solid bars), and pPVNGsKO mice (gray bars) (expressed as % control, n = 9–24/group). D, Quantitation of in situ hybridization for PVN Gsα expression in male and female mPVNGsKO mice and their controls. *, P < 0.05 vs. control; #, P < 0.05 vs. mPVNGsKO by one-way ANOVA.