Fig. 4.
Effect of PVN-specific Gsα deficiency on thermogenesis and cold tolerance. A, Food intake measured for 3.5 h after 200 μg MTII ip (left; expressed as % as that consumed after PBS injection) and % increase in energy expenditure after 10 μg/g MTII ip (right) in 5-month-old male mPVNGsKO and control mice (n = 6–10/group). B, Rectal temperature in 8- to 10-month-old mPVNGsKO, mBrGsKO, and control mice during exposure to 4 C environment (n = 5–7/group). C, BAT UCP1 mRNA expression in the same three groups of mice at room temperature (RT) and after 6 h of cold (4 C) exposure (n = 5–7/group). D, BAT norepinephrine (NE, left) and dihydroxyphenylglycol (DHPG, right) concentrations in male mPVNGsKO (solid bars) and control mice (open bars) (n = 6–8/group). E, Systolic blood pressure (left) and heart rate (right) in male mPVNGsKO, mBrGsKO, and control mice (n = 6–8/group). F, Gene expression in quadriceps muscles from mPVNGsKO and mBrGsKO mice and their respective control littermates (n = 4–6/group). PGC1, PPARγ coactivator 1α; mCPT, mitochondrial carnitine-palmitoyltransferase; CytoC, cytochrome C; Tfam, transcription factor A mitochondrial; Nrf1, nuclear respiratory factor 1. *, P < 0.05 vs. control by t test.