Figure 2.

Phylogenetic positioning of amoeba-resisting bacteria. The ribosomal RNA small subunit (16S) of Bacteria and two Archaea was aligned with Muscle and a neighbor-joining tree was subsequently built with Mega 5 (complete deletion, gamma distributed). The tree was rooted between Archaea and Bacteria. Bacterial phyla that comprise amoeba-resisting bacteria are indicated in green. Bacteria isolated from amoeba or growing within amoeba are highlighted in orange whereas those shown to resist amoebal phagocytosis in vitro are shown in blue. Finally other intracellular and fastidious bacteria are shown in purple.