Evolutionary relationship among HIV-1 sequences from Zhengzhou MSMs and other areas. Phylogenetic trees were constructed with the full length gag gene (A), pol gene (B), and partial env gene (C) of HIV-1 sequences from MSMs in Zhengzhou (●) and the reference sequences download from the Los Alamos National Laboratory (http://hiv-web.lanl.gov/), which showed the highest similarity to our strains. Each reference sequence is labeled with the HIV-1 subtype, followed by the isolated country and sequence name. The bootstrap probability (more than 70%, 1000 replica) was also indicated at the corresponding nodes of the tree. The scale bar representing unit genetic distance was labeled below. Reference strains from different areas, including Hubei province (▵), Hebei provinces (▴), Henan local area (▪), Liaoning province (□), Jiangsu province (○), Beijing (♦), Guangxi province (⋄), Heilongjiang province (▿), and Fujian province (▾), were labeled with different symbols.