Increasing ratio of in vivo neutrophil levels (n = 5, P < 0.05).
Notes: ▲: Original G-CSF (G-CSF:dextran = 1.4:5.0 ± 0.3 mg) solution; ⋄: G-CSF from prepared microspheres using the S/O/O/W method (PLGA 50/50 2A: 100.0 ± 0.5 mg PLGA, dextran nanoparticles [G-CSF:dextran, 1:4] = 10.0 ± 0.3 mg); ♦: G-CSF from control microspheres (prepared microspheres using the W/O/W method) (PLGA 50/50 2A: 100.0 ± 0.5 mg, G-CSF solution [G-CSF:dextran = 1:4] = 10.0 ± 0.3 mg).
Abbreviations: G-CSF, granulocyte colony–stimulating factor; PLGA, polylactic-co-glycolic acid; S/O/O/W, solid-in-oil-in-oil-in-water; W/O/W, water-in-oil-in-water.