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. 2010 Mar 18;44(1):42–51. doi: 10.2478/v10019-010-0010-3

FIGURE 1.

FIGURE 1

pp21-EGFP plasmid (A) and confirmation of successful construction of the plasmid (B). The identity of the plasmid was confirmed with SaII and EcoRI restriction. Sample 1 is WWP-Luc from which the p21 promoter (marked yellow) was isolated. Sample 2 is linearized pEGFP-N1 without CMV plasmid from which CMV was cut out with the same restriction enzymes to form blunt ends. Sample 3 is pp21-EGFP plasmid resulting from ligation of a 2.4 kB p21 promoter from WWP-Luc and sample 2 restricted with SaII and EcoRI.