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Loss of heterozygosity (LOH) of the RB1 gene at intron 17 (XbaI restriction polymorphism). Silver-stained 15% polyacrylamide gel. Lane 1: 50-bp DNA ladder (Fermentas, Vilnius, Lithuania); lane 2: heterozygous normal testis tissue; lane 3: LOH in the corresponding testicular germ cell tumor (nonseminoma, patient no. 25).
