TABLE 5.
Comparison of differential gene expression by microarrays and qRT-PCR for selected genes after bleomycin treatment, electroporation or electrochemotherapy with bleomycin
| gene |
fold-change ± st. dev.
|
treatment | |
|---|---|---|---|
| microarrays | qRT-PCR | ||
| up-regulated genesa | |||
| HSPA1B | 1,9 ± 0,1 | 2,0 ± 1,0 | EP |
| 1,6 ± 0,2 | 1,5 ± 0,5 | ECT BLM | |
| LASS2 | 1,5 ± 0,2 | 1,1 ± 0,6 | ECT BLM |
| PHLDA2 | 1,6 ± 0,1 | 2,0 ± 0,7 | ECT BLM |
| PRKCD | 1,5 ± 0,04 | 0,9 ± 0,4 | BLM |
|
| |||
| down-regulated genesa | |||
|
| |||
| VIM | 1,5 ± 0,1 | 1,5 ± 0,2 | ECT BLM |
|
| |||
| no change in expressiona | |||
|
| |||
| LAMB3 | 1b | 2,4 ± 0,7 | EP |
| PRKCD | 1b | 1,7 ± 0,1 | EP |
| PHLDA2 | 1b | 1,7 ± 0,2 | EP |
a
indicates change in expression as determined by microarrays.
b
indicates there was no change in gene expression determined by microarray analysis.