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. 2012 Jun 8;303(4):L279–L285. doi: 10.1152/ajplung.00361.2011

Fig. 4.

Fig. 4.

The attenuation of LPS-induced EC monocyte chemoattractant protein (MCP)-1 and regulated on activation normal T cell expressed and secreted (RANTES) expression by simvastatin is mediated by integrin-β4. EC were grown to confluence and treated with simvastatin or vehicle (5 μM, 16 h), followed by an integrin-β4-blocking antibody (ITGB4 ab, 20 μg/ml) or a control antibody (IgG) 2 h before LPS (500 ng/ml, 4 h). Media were collected and levels of MCP-1 (A) and RANTES (B) measured. Whereas pretreatment with the control antibody had no effect, pretreatment with an integrin-β4-blocking antibody reversed the attenuation of LPS-induced MCP-1 and RANTES expression by simvastatin (n = 3/condition, *P < 0.05, **P < 0.05 compared with LPS alone).