Fig. 6.

Protection by simvastatin in murine ALI is attenuated by inhibition of integrin-β4 as assessed by Evans blue dye albumin (EBA) extravasation and lung histology. Mice were administered simvastatin (20 mg/kg, 24 h) or vehicle intraperitoneally followed by intravenous integrin-β4-blocking antibody (ITGB4, 1 mg/kg) or a control antibody (IgG, 1 mg/kg) 4 h before concomitant treatment with a second dose of simvastatin and LPS (1.25 mg/kg, intratracheally). A: in select animals, EBA was injected and lung tissue extravasation was measured (n = 3 animals/group, *P < 0.05 compared with controls, **P < 0.05 compared with LPS alone, †P < 0.05). B: in separate experiments, lungs were collected for histology (representative images shown).