Fig. 5.

Increased pIκBα-NF-κB signaling during MV-O₂ is suppressed in elafin-expressing mice. A: immunoblots showing increased pIκBα protein relative to total IκBα protein in cytoplasmic extracts of lungs obtained from 6-day-old wild-type mice after MV-O₂ for 24 h, compared with unventilated controls that breathed 40% O₂ for 24 h (left). pIκBα protein was unchanged in response to MV-O₂ in cytoplasmic extracts of lungs obtained from elafin-expressing mice compared with unventilated controls (right). B: immunoblots showing suppression of NF-κB-p65 protein relative to β-actin protein in nuclear extracts of lungs obtained from 6-day-old elafin-expressing mice after MV-O₂ for 24 h, compared with unventilated controls that breathed 40% O₂ for 24 h (right). NF-κB-p65 protein did not change significantly in nuclear extracts of lungs obtained from wild-type mice after MV-O₂ for 24 h (left). *Significant difference compared with Control, P < 0.05. Values are means and SD; n = 3–5/group.