FIG. 2.

HIF-1α-dependent induction of miR-107 improved ^PCMSC survival. (A) Western blot showing the successful abrogation of HIF-1α in the nuclear fraction of ^PCMSC with PCx2 and transfected with HIF-1α siRNA as compared with Sc siRNA-transfected ^PCMSC. Native ^non-PCMSC were used as controls. (B) RT-PCR showing the abrogation of miR-107 in ^PCMC (PCx2) in response to HIF-1α siRNA transfection as compared with Sc siRNA-transfected ^PCMSC. (C) The abrogation of HIF-1α led to the loss of cytoprotection in ^PCMSC as determined by LDH-release assay. ^PCMSC (both PCx1 and PCx2) with HIF-1α siRNA transfection had a significantly higher release of LDH under 6 h of lethal anoxia as compared with ^ScMSC. (D) Representative fluorescence images and quantitative analysis of TUNEL positivity (green) showed that the abrogation of HIF-1α significantly increased TUNEL-positive ^PCMSC as compared with Sc siRNA-transfected ^PCMSC. DAPI was used to visualize nuclei. HIF-1α, hypoxia inducible factor-1α; N.S., non-significant; RT-PCR, reverse transcription polymerase chain reaction; Sc, scramble; TUNEL, transferase-mediated dUTP nick-end labeling. (To see this illustration in color the reader is referred to the web version of this article at www.liebertpub.com/ars).