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. 2012 Oct 15;17(8):1053–1065. doi: 10.1089/ars.2012.4518

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 8. — ^PCMSC had improved survival in the infarcted rat heart. (A) PCR for sry-gene expression in the female animal hearts from different treatment groups on day 4 after their respective treatment. Sry-gene expression was significantly higher in the ^PCMSC-transplanted animal hearts of group-3 as compared with the ^Non-PCMSC-transplanted animal hearts of group-2. The DMEM-treated animal hearts of group-1 did not show the sry-gene and served as a negative control. (B) qRT-PCR showing a higher level of expression of miR-107 in the infarcted rat heart on day 4 post-transplantation of ^PCMSC as compared with ^Non-PCMSC and DMEM-treated animal hearts. (C) PCR for the sry-gene analysis of animal hearts transplanted with ^PCMSC trnasfected with anti-miR-107 and anti-miR-210 using Sc-transfected ^PCMSC as a control. Transfection with anti-miR-107 and anti-miR-210 significantly abolished the cytoprotective effects of preconditioning. ^PCMSC with anti-miR-107 (animal group-5) and anti-miR-210 (animal group-6) transfection showed significantly lower survival as compared with the Sc-transfected ^PCMSC (animal group-4). DMEM, Dulbecco's Modified Eagle Medium.