FIG. 4.

A representative autoradiogram using various concentrations of PF1 in the RT inhibition assay. (A) Schematic diagram of the primer (black)-template (gray) used to conduct the reverse transcriptase (RT) inhibition assay. The primer strand was radiolabeled with ³²P at the 5′ end. (B) The assay was conducted by incubating the prehybridized primer–template (50 nM) with 100 μM dNTPs and various concentrations of aptamers as indicated. Reactions were initiated with HIV RT (0.25 nM), and aliquots were removed at 2, 5, 10, 15, and 20 minutes. The samples were run on a denaturing 10% polyacrylamide gel and quantified as described in Materials and Methods.