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. Author manuscript; available in PMC: 2012 Aug 21.
Published in final edited form as: Nat Cell Biol. 2008 Nov 16;10(12):1470–1476. doi: 10.1038/ncb1800

Figure 3. Glioblastoma microvesicles can deliver functional RNA to HBMVECs.

Figure 3

(a) Purified microvesicles were labelled with membrane dye PKH67 (green) and added to HBMVECs. The microvesicles were internalised into endosome-like structures within an hr. (b) Microvesicles were isolated from glioblastoma cells stably expressing Gluc. RNA extraction and RTPCR of Gluc and GAPDH mRNAs showed that both were incorporated into microvesicles. (c) Microvesicles were then added to HBMVECs and incubated for 24 hrs. The Gluc activity was measured in the medium at 0, 15 and 24 hrs after microvesicle addition and normalized to Gluc activity in microvesicles. The results are presented as the mean ± SEM (n = 4).