Table 1. RNA in glioblastoma microvesicles can be used as sensitive biomarkers.

Nested RT-PCR was used to monitor EGFRvIII mRNA in glioma tissue and exosomes purified from a frozen serum sample from the same patient. Samples from 30 patients were analysed in a blinded fashion and PCR reactions were repeated at least three times for each sample. No EGFRvIII mRNA was found in serum microvesicles from 30 normal controls.

Patient#	Time of serum collection*	Serum volume	Biopsy EGFRvIII	Serum exosome EGFRvIII
1	0	3 ml	Yes	Yes
2	0	2 ml	No	No
3	0	2.5 ml	No	No
4	0	1 ml	Yes	No
5	0	1 ml	Yes	No
6	0	1 ml	No	No
7	0	0.6 ml	Yes	Yes
8	0	1 ml	No	No
9	0	1 ml	Yes	Yes
10	0	1 ml	No	Yes
11	0	2 ml	Yes	No
12	0	2 ml	Yes	Yes
13	0	2 ml	No	Yes
14	0	2 ml	Yes	Yes
15	0	2 ml	No	No
16	0	2 ml	No	No
17	0	1 ml	Yes	No
18	0	0.8 ml	Yes	No
19	0	1 ml	No	No
20	0	1 ml	No	No
21	0	1 ml	No	No
22	0	1 ml	No	No
23	0	1 ml	No	No
24	0	1 ml	No	No
25	0	1 ml	No	No
26	14	0.6 ml	Yes	No
27	14	1.2 ml	No	No
28	14	0.8 ml	Yes	No
29	14	0.9 ml	Yes	No
30	14	0.6 ml	Yes	No

^*Days post-surgery