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. Author manuscript; available in PMC: 2013 Aug 10.
Published in final edited form as: Vaccine. 2012 Jul 6;30(37):5453–5458. doi: 10.1016/j.vaccine.2012.06.074

Figure 1. Deletion/insertion strategy for producing rubella vectors.

Figure 1

(A) Rubella genome, showing nonstructural proteins P150 and P90, structural proteins capsid, E2 and E1, and the genomic (Pgen) and subgenomic (Psub) promoters. A deletion between two Not I restriction sites in P150 allowed insertion of foreign genes at the same site. (B) HIV-1 gp41 inserts contained one or two epitopes of the membrane-proximal external region (MPER) targeted by broadly neutralizing antibodies 2F5 and 4E10. SIV Gag amino acids 41 to 211 contain five T cell epitopes, labeled A through E. The epitopes in each construct are shown schematically. In constructs denoted by an asterisk (*) one epitope at a time was added to the carboxyl end of sGag2L to produce sGag2L-A, -B, or -C. The vector BC-sGag2 contained four epitopes and replicated strongly for at least eight passages. Vector replication after 2 to 8 passages was detected with antibodies to the rubella structural proteins C and E1 and is indicated by + or −.