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Titers of rubella vector stocks of potential vaccine candidates.
| Viral vector | RNA copies/ml | Estimated titer, PFU/ml |
|---|---|---|
| MPERF in NotI site (P6) | 3.1 × 107 | 5.0 × 106 |
| BC-sGag2 in NotI site (P6) | 2.5 × 106 | 4.1 × 105 |
The viral RNA content of each vector was determined by real time RT-PCR. The titer was estimated by comparison to a vaccine standard of known infectious titer. Insert sequences were verified by viral genome sequencing.
