Table 1.
Oligonucleotide primers employed.
Gene | Primer sequence | Tm °C | Amplicon size (bp) | |
---|---|---|---|---|
9s rRNA | F | 5′-ATCCGCCAACGTCACATT-3′ | 57.6 | 115 |
R | 5′-CCGCCGCCATAAGGAGAAC-3′ | 64.5 | ||
CD36 | F | 5′-CAGTCGGAGACATGCTTATTGAG-3′ | 60.7 | 151 |
R | 5′-TTTGCCACGTCATCTGGGTTT-3′ | 62.5 | ||
COX-2 | F | 5′-TGTGACTGTACCCGGACTGG-3′ | 63.0 | 233 |
R | 5'-TGCACATTGTAAGTAGGTGGAC-3′ | 60.0 | ||
FABP4 | F | 5′-GGGGCCAGGCTTCTATTCC-3′ | 61.8 | 114 |
R | 5′-GGAGCTGGGTTAGGTATGGG-3′ | 61.1 | ||
GAPDH | F | 5′-GGACGCATTGGTCGTCTGG-3′ | 63.0 | 204 |
R | 5′-TTTGCACTGGTACGTGTTGAT-3′ | 60.2 | ||
IL-12 p40 | F | 5′-CAAGGCTGTTCACATTATCCCA-3′ | 60.3 | 107 |
R | 5′-CCAGTGTGGTCATGGACTTTC-3′ | 60.6 | ||
MCP-1 | F | 5′-TTAAAAACCTGGATCGGAACCAA-3′ | 60.1 | 121 |
R | 5′-GCATTAGCTTCAGATTTACGGGT-3′ | 60.7 | ||
NOS2 | F | 5′-ACATCGACCCGTCCACAGTAT-3′ | 62.7 | 177 |
R | 5′-CAGAGGGGTAGGCTTGTCTC-3′ | 61.0 | ||
NOX4 | F | 5′-TGCCTGCTCATTTGGCTGT-3′ | 62.2 | 180 |
R | 5′-CCGGCACATAGGTAAAAGGATG-3′ | 60.8 | ||
PPAR-γ | F | 5′-CCATTCTGGCCCACCAAC-3′ | 66.5 | 479 |
R | 5′-CTGAAACCGACAGTACTG-3′ | 53.8 | ||
TNF-α | F | 5′-CCTGTAGCCCACGTCGTAG-3′ | 61.5 | 148 |
R | 5′-GGGAGTAGACAAGGTACAACCC-3′ | 61.4 |