Table 2.
Effect of CUR and 5/6NX on mitochondrial oxygen consumption using malate/glutamate or succinate as substrates.
| Control | 5/6NX | 5/6NX + CUR | CUR | |
|---|---|---|---|---|
| Malate/glutamate | ||||
| State 3 (ngAO/min/mg) | 193.9 ± 19.2 | 197.3 ± 7.3 | 187.9 ± 12.8 | 188.8 ± 17.5 |
| State 4 (ngAO/min/mg) | 72.6 ± 8.7 | 77.7 ± 6.4 | 69.7 ± 4.3 | 72.9 ± 7.1 |
| RC | 2.8 ± 0.2 | 2.5 ± 0.1 | 2.7 ± 0.1 | 2.6 ± 0.1 |
| Uncoupled respiration (ngAO/min/mg) | 233.1 ± 28.0 | 238.3 ± 22.7 | 225.3 ± 19.5 | 247.1 ± 28.3 |
| ADP/O | 2.2 ± 0.4 | 1.8 ± 0.1 | 1.9 ± 0.1 | 2.1 ± 0.2 |
|
| ||||
| Succinate | ||||
| State 3 (ngAO/min/mg) | 277.1 ± 31.3 | 276.1 ± 20.7 | 277.1 ± 14.8 | 251.1 ± 24.2 |
| State 4 (ngAO/min/mg) | 136.4 ± 14.0 | 143.0 ± 13.2 | 136.1 ± 5.3 | 114.1 ± 0.7 |
| RC | 2.0 ± 0.1 | 2.0 ± 0.1 | 2.0 ± 0.1 | 2.2 ± 0.1 |
| Uncoupled respiration (ngAO/min/mg) | 414.5 ± 49.2 | 340.7 ± 53.3 | 399.7 ± 26.8 | 425.0 ± 49.2 |
| ADP/O | 1.3 ± 0.1 | 1.2 ± 0.0 | 1.2 ± 0.1 | 1.4 ± 0.1 |
CUR: curcumin, 5/6NX: five-sixths nephrectomy, RC: respiratory control index, ngAO/min/mg: nanograms atoms oxygen/minute/milligrams protein. ADP/O: phosphorylation efficiency. Data represent mean ± SEM, n = 7–15.