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. 2012 Aug 7;2012:390385. doi: 10.1155/2012/390385

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Copyright © 2012 Carlota Oleaga et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of cyclin D1 upon incubation with ICC and CA in HT29 and MCF-7 cells. (a) Quantitation of STAT5b (empty bars), ATF-2 (filled bars), and cyclin D1 (grey bars) protein levels in MCF-7 cells. The protein levels were determined in control MCF-7 cells (CNT) and cells treated with caffeic acid (CA) and instant coffee (ICC) by Western blot. Blots were reprobed with an antibody against β-actin to normalize the results. Results represent the mean ± SE of 3 different experiments. *P < 0.05 and ***P < 0.001 compared with the corresponding control. (b) Quantitation of STAT5b (empty bars), ATF-2 (filled bars), and cyclin D1 (grey bars) protein levels in HT29 cells. The protein levels were determined in control HT29 cells (CNT) and cells treated with caffeic acid (CA) and instant coffee (ICC) by Western blot. Blots were reprobed with an antibody against β-actin to normalize the results. Results represent the mean ± SE of 3 different experiments.*P < 0.05 and **P < 0.01 compared with the corresponding control.