Fig. 3.

Autopod and zeugopod phenotypes of Gli3;Neo1 double mutants. A: Bone and cartilage staining of autopods of E18.5 mice of the indicated genotypes. Numbers on digits represent their identity. The asterisk indicates a nubbin. B: Bone and cartilage staining of limbs of E18.5 embryos of the indicated genotypes. The upper and the lower rows represent forelimbs and hindlimbs, respectively. Note the absence of a radius in the Gli3^Xt/Xt;Neo1^Gt/Gt embryo (black arrow) and the rudimentary and absent tibias in the Gli3^Xt/Xt;Neo1^+/+ and Gli3^Xt/Xt ;Neo1^Gt/Gt embryos, respectively (red arrowheads). The asterisk represents reduced bone formation in the anterior stylopod of the Gli3^Xt/Xt;Neo1^Gt/Gt embryo. Wt, wild-type; H, humerus; R, radius; U, ulna; Fe, femur; T, tibia; Fi, fibula.