Fig. 5.

Intracellular [Cl⁻] and E_Cl are independent of DRG cell phenotype. A: basal [Cl⁻]_i in IB4⁺ and IB4⁻ DRG neurons. Average basal [Cl⁻]_i was 51 ± 19 mM (n = 117 cells; mean ± SD) in IB4⁺ neurons and 54 ± 18 mM (n = 59 cells) in IB4⁻ neurons. The difference between means was not significant (P = 0.27). B: E_Cl in IB4⁺ (−28 ± 13 mV) and IB4⁻ (−26 ± 13 mV) cells (mean ± SD). The difference between means was not significant (P = 0.44). C: scatterplot of [Cl⁻]_i and soma area in IB4⁺ and IB4⁻ DRG neurons. There was no correlation between [Cl⁻]_i and soma area (r = 0.06). D: scatterplot of E_Cl and soma area in IB4⁺ and IB4⁻ DRG neurons. The gray shaded rectangular area represents the reported average range of resting membrane potential (E_m; −55 to −65 mV). E_Cl was less negative than E_m in most cells (95.5%); E_Cl was equal to E_m in ∼2% of the cells; and E_Cl was more negative than E_m in only ∼3% of the cells. (HEPES-buffered solutions, [Cl⁻]_o = 131 mM; error bars in A and B represent SD).