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. 2012 Mar 28;108(3):834–852. doi: 10.1152/jn.00970.2011

Fig. 8.

Fig. 8.

Expression of KCC1, KCC3, and KCC4 mRNAs in neonate and adult rat DRG. A: schematic diagram of the primers designed to detect KCCs other than KCC2 in rat DRG, kidney, and brain tissues. The KCC1 primer set (KCC1-520) is predicted to amplify all KCC1 transcripts from the mRNA region encompassing the 3′-end of exon 1 and 3′/5′ boundaries of exons 5 and 6. The KCC3-547 primer set amplifies all predicted KCC3 isoforms and splice variants, and the primer KCC4-665 amplifies predicted rat KCC4 cDNA sequences (see materials and methods for details). B and C: expression of KCCs in rat DRG, kidney, and brain in neonate (P0, B) and adult (P28 and P17, C) rat. Control lane in B and C was water instead of RNA.