Fig. 9.

Expression of KCC3 splice variants in neonate and adult rat DRG. A: schematic diagram of the primers designed to detect KCC3 isoforms and splice variants in rat tissues. The primer KCC3-662a/617a⁻² was designed to selectively detect the “a” variants of KCC3, with or without exon 2 (662 bp or 617 bp, respectively). The primer set KCC3-556b/511b⁻² was designed to specifically detect the “b” variants of KCC3 with or without exon 2 (556 bp or 511 bp, respectively). B: KCC3 transcript expression in neonate (P0) and juvenile (P17) rat DRG and brain. Note that DRG expresses at least 3 KCC3 splice variants.