Figure 1.

Splice variants in durum wheat SBEIIa_B(SJ12^d) mutant. (A) Gene models for genomic sequences of exons 11–14 of SBEIIa_A (blue) and SBEIIa_B (green) are shown (boxes represent exons and lines represent introns). The splice junction mutation in the SBEIIa_B gene is located at the end of exon 12 in the splice donor site as indicated in red in the sequence and with the red symbol. The locations of PCR primers used to amplify cDNA for evaluation of splice variants are shown with arrows. (B) Graphical representation of cloned cDNA made from RNA of a wild-type sibling line. The numbers of SBEIIa transcripts derived from either the A (blue) or B (green) genomes are indicated. (C) Graphical representation of cloned cDNA made from RNA of B genome splice junction mutant homozygous line. The number and type of alternatively spliced cDNAs from either the A (blue) or B (green) genomes are indicated.