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. 2012 Aug 21;7(8):e41877. doi: 10.1371/journal.pone.0041877

Figure 4. scN attenuates transcriptional changes induced by bergapten.

Figure 4

Selected bergapten- and scN-coregulated genes involved in polysaccharide or protein degradation (CmGH5, CmCatLs, CmCatB), detoxification (CmCYP6G1s, CmGST, CmPOD), defense (CmDrsL1-1), development (CmJHE7) and transport (CmSUT1) were subjected to qPT-PCR analyses. Total RNA was extracted from midgut of the 4th instar larvae feeding on artificial diet containing 1,000 ppm scN, 250 ppm bergapten or 1,000 ppm scN + 250 ppm bergapten, respectively. Insects feeding on diet without bergapten or scN served as the control. Reverse transcription and qRT-PCR reactions were performed as described in Material and Methods. Transcript fold induction derived from qRT-PCR is shown as bar graphs. The lower panel shows microarray results of the corresponding genes. “+”, “−”: up- or down-regulation when subjected to scN or bergapten treatment. CmGH5, Glycoside hydrolase; CmCatLa and CmCatLb, cathepsin L-like proteases; CmCatB, cathepsin B-like protease; CmCYP6G1-2 and CmCYP6G1-3, Cytochrome P450s; CmGST, Glutathione S-transferase; CmDrsL1-1, Drosomycin-like I; CmJHE7, Juvenile hormone esterase; CmSUT1, Sugar transporter 1; CmPOD, Peroxidase precursor.