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Contribution of the RocR regulon to amylase production.B. subtilis MGB874 derivative strains in the presence (+) or absence (-) of the indicated genes were transformed with pHYK38, and then cultured in 2xL-Mal medium with shaking at 30°C. α-amylase activities were measured after 72 h cultivation. The following strains were evaluated: (a), MGB874; (b), 874DEFR; (c), 874RocR; (d), 874DEFRΔrocG; and (e), 874DEFRΔrocABC.
