Table 2.
Strain | Plasmid | Na2CO3a | α-amylase activity(kU l-1)b |
---|---|---|---|
168 |
pHYK38 |
- |
2.9 ± 0.3 |
168ΔdltB |
pHYK38 |
- |
7.0 ± 0.3 |
MGB874 |
pHYK38 |
- |
0.10 ± 0.01 |
874ΔdltB |
pHYK38 |
- |
0.27 ± 0.02 |
168 |
pHYK38 |
+ |
14.3 ± 0.2 |
168ΔdltB |
pHYK38 |
+ |
14.8 ± 0.4 |
MGB874 |
pHYK38 |
+ |
20.8 ± 0.8 |
874ΔdltB | pHYK38 | + | 20.1 ± 0.2 |
aB. subtilis derivative strains were grown in 2xL-Mal medium at 30°C. At the transition phase (12 h), an Na2CO3 solution was added to the growth medium at a final concentration of 0.9% (w/v). -, No addition; +, Addition.
bα-amylase activity was determined after 72 h of cultivation.