Table 3.
Bacterial strains and plasmids used or constructed in this study
| Strain or plasmid | Relevant properties† | Source or reference |
|---|---|---|
| Strain |
|
|
|
Bacillus subtilis |
|
|
| 168 |
trpC2 |
[5] |
| MGB625 |
MGB604 Δ(yeeK-yesX) |
[8] |
| MGB723 |
MGB625 Δ(pdp-rocR) |
[8] |
| MGB874 |
MGB860 Δ(yncM-yndN) |
[8] |
| MGB625Δr1 |
MGB625 Δ(pdp-yxeD)::cat |
[11] |
| MGB625Δr2 |
MGB625 Δ(yxeC-yxnA)::cat |
[11] |
| MGB625Δr3 |
MGB625 Δ(yxaD-fbp)::cat |
[11] |
| MGB625Δr4 |
MGB625 Δ(yydD-rocR)::cat |
[11] |
| MGB625ΔyydD-yycN |
MGB625 Δ(yydD-yycN)::cat |
[11] |
| MGB625ΔrapG-phrG |
MGB625 Δ(rapG-phrG)::cat |
[11] |
| MGB625ΔrocF-rocR |
MGB625 Δ(rocF-rocR)::cat |
[11] |
| MGB625Δpdp-rocD |
MGB625 Δ(pdp-rocD)::cat |
This study |
| 874DEFR |
MGB874 cat-rocDEF-rocR was inserted at the Δ(pdp-rocR) locus |
[11] |
| 874RocR |
MGB874 cat -rocR was inserted at the Δ(pdp-rocR) locus |
This study |
| 874DEFRΔrocG |
874DEFR ΔrocG::spec |
[11] |
| 874DEFRΔrocABC |
874Δ DEFRΔrocABC::spec |
This study |
| R-726 |
MGB874 ΔaprE::Pspac-rocG spec (pAPNC213-rocG) |
This study |
| R-1645 |
168 ΔamyE::PhtrB-lacZ cat (pDN110) |
This study |
| R-1646 |
MGB874 ΔamyE::PhtrB-lacZ cat (pDN110) |
This study |
| R-1926 |
MGB874 ΔaprE::Pspac-rocG spec ΔamyE::PhtrB-lacZ cat (pDN110) |
This study |
| 168ΔcssRS |
168 ΔcssRS::spec |
This study |
| 874ΔcssRS |
MGB874 ΔcssRS::spec |
This study |
| 168ΔdltB |
trpC2 ΔdltB::neo |
This study |
| 874ΔdltB |
MGB874 ΔdltB::neo |
This study |
|
Escherichia coli |
|
|
| HB101 |
supE44 Δ(mcrC-mrr) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1 |
Takara Bio |
| Plasmid |
|
|
| pHY300PLK |
Shuttle vector for E. coli and B. subtilis |
Takara Bio |
| pHYK38 |
pHY300PLK carrying the gene for AmyK38 from Bacillus sp. strain KSM-K38 fused to the promoter, SD, and leader peptide sequences of egl-237, containing amp and tet |
This study |
| pAPNC213 |
The integration vector containing Pspac promoter into the aprE locus by double crossover using spec |
[35] |
| pAPNC213-rocG |
pAPNC213 containing the SD sequence and coding region of rocG (−25 to 1275, 1325 bp) |
This study |
| pDL2 | Integration vector for introduction of single-copy transcriptional fusions to lacZ by double crossover at the amyE locus (cat) | [36] |
†Antibiotic resistance genes are abbreviated in the table as follows: amp, ampicillin; tet, tetracycline; cat, chloramphenicol; spec, spectinomycin; and neo, neomycin. SD, Shine-Dalgarno.