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. 2012 Aug 21;7(8):e43372. doi: 10.1371/journal.pone.0043372

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© 2012 Iwasaki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Micro-computed tomography of the cervical spine of Enpp1^ttw/ttw mice at 12 weeks of age with or without Runx2 haploinsufficiency. 3D reconstructed images.Ectopically calcified region is shown in yellow. B, Quantitative analysis of the ossification of the cruciform ligament at the atlanto-occipital area using micro-CT in Enpp1^ttw/ttw mice at 8 weeks of age with or without Runx2 haploinsufficiency. Note a significant decrease in calcified region in ENPP1^ttw/ttw/Runx2^+/− mice. C,D Micro-CT analysis of the ossification of the cruciform ligament at the atlanto-occipital area in Enpp1^ttw/ttw mice at 12 weeks of age with or without Runx2 haploinsufficiency. Bone mineral content (C) Bone mineral density (D).