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. 2012 Aug 21;7(8):e43468. doi: 10.1371/journal.pone.0043468

Figure 2. eIF-5A, DHS and DOHH are overexpressed in the glioblastoma cell lines G55T2 and U87-MG.

Figure 2

(A) Expression levels of eIF-5A, -A2, DHS and DOHH were analysed by qPCR (mean ±SD, n = 3; *:P<0.05; **:P<0.001 ) in primary normal human astrocytes (NHA) compared to G55T2 and U87-MG cell lines. SYBR green Ct values were normalized against GAPDH expression and calculated using the 2−ΔΔCT method. (B) Overexpression of eIF-5A, DHS and DOHH was confirmed by immunoblotting with whole cell lysates (35 µg protein per sample).