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. 2012 Aug 21;7(8):e43748. doi: 10.1371/journal.pone.0043748

Figure 6. Electroporation of Oct4-specific shRNA expression vectors.

Figure 6

(A) Blastocyst development following electroporation of zygotes with custom Oct4-specific shRNA expression vectors at different concentrations and cultured for 3.5 d. (B) Blastocyst development following electroporation of zygotes with different shRNA expression vectors with a concentration of 100 µg/ml. (C) Morphological appearance of electroporated zygotes obtained from the control and Oct4-shRNA groups after being cultured for 3.5 d. Morphology (top) and fluorescence (bottom). The original magnification was ×100.