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. 2012 Aug 21;7(8):e43748. doi: 10.1371/journal.pone.0043748

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© 2012 Peng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Blastocyst development following electroporation of zygotes with customized Oct4 morpholinos at different concentrations and cultured for 3.5 d. (B) Blastocyst development following electroporation of zygotes with different morpholinos with a concentration of 0.4 mM. (C) Morphological appearance of electroporated zygotes obtained from the control and Oct4-MOs groups after being cultured for 3.5 d. The original magnification was ×100. (D) Nuclear Oct4 expression is absent in Oct4-MOs electroporated embryos but present in embryos obtained from the control groups. Each sample was counterstained with Hoechst 33342 to visualize DNA (blue). The original magnification was ×200.