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. Author manuscript; available in PMC: 2012 Sep 23.
Published in final edited form as: Immunity. 2011 Aug 11;35(3):388–399. doi: 10.1016/j.immuni.2011.07.007

Figure 1. Skin Irritant-Induced DC Migration to Cutaneous DLNs Is Impaired in Eps8 Null Mice.

Figure 1

(A) The percentage of FITC+ MHCII+ skin cells migrating to inguinal DLNs of WT (Eps8+/+) or Eps8−/− (Eps8−/−) mice painted on the abdomen with 0.5% FITC in 1:1 acetone:dibutylphtalate mixture was assessed by flow cytometry. Numbers in the gate are the percentage of FITC+ MHCII+ positive cells (6 mice/group, two independent experiments).

(B) The absolute numbers of FITC+ MHCII+ CD11c+ cells migrating to the DLNs at different time points in WT (Eps8+/+) and Eps8-deficient (Eps8−/−) animals. Data represent the mean ± SD (6 mice/group, out of two independent experiments).

(C)FITC+MHCII+CD11c+ cells were analyzed for the expression of CD24 and CD207. Numbers in the quadrants show the percentage of positive cells.

(D) The absolute numbers of FITC+ dermal DCs (CD24− CD207−), langerin dermal DCs (CD24− CD207+) and Langerhans cells (CD24+ CD207+) migrating to the DLNs at different time points in WT (Eps8+/+) and Eps8-deficient (Eps8−/−) animals. Data represent the mean ± SD (n = 6 mice/group of two independent experiments); *p < 0.05; **p < 0.01; ***p < 0.001.