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. Author manuscript; available in PMC: 2012 Sep 23.
Published in final edited form as: Immunity. 2011 Aug 11;35(3):388–399. doi: 10.1016/j.immuni.2011.07.007

Figure 4. Genetic Removal of Eps8 Impairs Directional Motility of BMDCs in Under-Agarose Assays.

Figure 4

(A) An equal number of LPS-activated WT (Eps8+/+) and Eps8−/− (Eps8−/−) BMDCs were injected between a glass coverslip and an agarose layer. Cells were induced to migrate toward a 2mm afar attractor hole containing CCL19. Migration tracks of randomly picked cells are shown on the left (see also Movie S1). The forward migration index and mean velocity (right graphs) ± SD are shown. At least 30 single cells /experiment/genotype were tracked. n.s. not significant; **p < 0.01.

(B) Random BMDC migration in a planar under-agarose assay. BMDCs were treated as in (A) and injected between a glass coverslip and a layer of agarose. Data are presented as in (A) except that the directionality index is reported instead of forward migration index (right graphs). (see also Movie S2). At least 30 single cells/experiment/genotype (three independent BMDC preparations) were tracked. Data are the mean ± SD. n.s. not significant; *p < 0.05.