(A) An equal number of LPS-activated WT (Eps8+/+) and Eps8−/− (Eps8−/−) BMDCs were placed on one side of a chamber slide in which 1.6 mg/ml polymerized collagen type I gel containing 1.2 µg/ml CCL19 was added (Friedl and Bröcker, 2000). A time sequence of migratory WT and Eps8−/− BMDCs is shown (see also Movie S3). The scale bar represents 150 µm.
(B and C) Migration tracks of randomly picked individual cells (B). Cell directionality (C, left graph) and mean velocity (C, right graph) were measured. The analysis was conducted on 35 single cells/experiment/genotype of three independent BMDC preparations; data are the mean ± SD. **p < 0.01.