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. Author manuscript; available in PMC: 2012 Aug 22.
Published in final edited form as: Biochemistry. 2009 Nov 24;48(46):10997–11010. doi: 10.1021/bi9008374

Table 2.

Dependence of E(Q)his Yield on the Binding of Monovalent Cations and on the α-Site Ligand, F9a,.

Estimated Yield of E(Q)his (%)a
MVC −F9 +F9
MVC-free ~1.5 ~16.0
Na+ ~2.0 ~18.2
NH4+ ~14.0 100b
Cs+ ~17.3 ~89
a

Percentages estimated from spectra in Figure 6.

b

The yield obtained with the NH4+ form of the F9 complex was assumed to be 100% of the β-sites.